DIY Bio Potato Dextrose Agar

Agar pour

Ingredients for 250mL:

  • 2.5g dehydrated potato flakes (I used Homebrand)
  • 5g dextrose
  • 10g agar, I used the Thai fish brand from the Chinese food store
  • 250mL tap water

Equipment:

  • tinfoil
  • pyrex jug
  • microwave
  • autoclave tape

Method:

Weigh out dry ingredients into microwaveable pyrex jug, add tap water to 250mL.

Microwave on high for 2-5min, until mixture boils and agar is dissolved.

Pour into glass petri dishes immediately, filling the dishes about half full and put lids back on dishes.

Wrap petri dishes containing agar in tin foil, mark with autoclave tape.

Sterilise using the pressure cooker method.

Store wrapped at 4 degrees celsius for up to a week.